Homo Sapiens (Human) Primers (used in Sanger sequencing)

Ig heavy chain
Nest PCR or half-nest PCR

First round:
VH1 (5'-GAGGTGCAGCTCGAGGAGTCTGGG-3'),
VH2 (5'-CAGGTGCAGCTCGAGCAGTCTGGG-3'),
VH3 (5'-CAGGTACAGCTCGAGCAGTCAGG-3'),
VH4 (5'-CAGGTGCAGCTGCTCGAGTCGGG-3'),
Cμ-CH1, 5'-ACGCTGC TCGTATCCGACGGG-3'
Or CγCH1, 5'-ACGCTGCTCGTATCCGACGGG-3'

Second round:
FR2 coupled with JH primer was used
FR2: 5’-TGG(A/G)TCCG(A/C/G)CAG(G/C)C(T/C)CC(A/C/G/T)GG-3’;
TGGRTCCGVCAGSCYCCNGG
JH: 5’-AACTGCAGAGGAGACGGTGACC-3’
or JH2: 5’-GTGACCAGGGTNCCTTGGCCCCAAG-3'

Ig kappa chain

Vκ1 (5’-GACATCGAGCTCACCCAGTCTCC-3’) and
Vκ 2 (5’-GAAATTGAGCTCACGCAGTCTCCA-3’)

Cκ2 primer (Cκ2: 5’-CGGGAAGATGAAGACAGATGGTGC-3’)
Half-nest PCR
Cκ1 primer (5’-TGGTGCAGCCACAGTTCGTTT-3’)
Or
JK1/2/3/4 : 5’-CTTACGTTTGATCTCCACCTTGGTCCC-3’
JK5 : 5’-CTTACGTTTAATCTCCAGTCGTGTCCC-3’